Rapid Emergence of a Viral Resistant Mutant in Whv Chronically Infected Woodchucks Treated with Lamivudine and a Pre-s/S Cho-derived Hepatitis B Virus Vaccine.
From: Viral Hepatitis Unit, Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
Vaccine
- Publish Date: Jun 2007
- ISSN: 0264-410X
- Volume: 25
- Issue: 26
- Pages: 4895-902
- Medium: Print
- Language: English
- Citation (JAMA): D'Ugo Emilio, Kondili Loreta A, Canitano Andrea, et al. Rapid Emergence of a Viral Resistant Mutant in Whv Chronically Infected Woodchucks Treated with Lamivudine and a Pre-s/S Cho-derived Hepatitis B Virus Vaccine.. Vaccine Jun 2007;25:4895-902
Abstract
To determine whether the addition of a pre-S/S human vaccine increases the antiviral activity of lamivudine, four woodchucks were treated with a daily dose of 100 mg/kg lamivudine and four 50 microg doses of CHO-derived pre-S/S human vaccine. WHV DNA titres decreased up to two logarithms in three woodchucks. At week 4, in three of the animals, the sequence analysis showed a predominant strain containing a nucleotide change from A to T at position 1696 of domain B of the WHV DNA polymerase. Vaccination did not further suppress WHV DNA, despite anti-HBs production in three animals. The woodchuck remains a useful model for characterising the biology and kinetics of the emergence of drug-resistant variants and could be used for pre-clinical studies of combinations of new antiviral drugs.
Mesh Headings (Keywords): Animals, CHO Cells, Cricetinae, Cricetulus, DNA, Viral, Drug Resistance, Viral, Hepatitis B, Hepatitis B Vaccines, Hepatitis B Virus, Woodchuck, Inflammation, Kinetics, Lamivudine, Liver, Marmota, Monocytes, Mutation, Reverse Transcriptase Inhibitors, Reverse Transcriptase Polymerase Chain Reaction, Vaccination
Check for Full Text / PubMed Unique Identifier (PMID): 17531355
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