Marlin-1 and Conventional Kinesin Link Gabab Receptors to the Cytoskeleton and Regulate Receptor Transport.
From: Physiology and Biophysics, ICBM, Faculty of Medicine, Universidad de Chile Independencia 1027, Santiago, Chile.
Molecular and cellular neurosciences
- Publish Date: Jul 2007
- ISSN: 1044-7431
- Volume: 35
- Issue: 3
- Pages: 501-12
- Medium: Print
- Language: English
- Citation (JAMA): Vidal René L, Ramírez Omar A, Sandoval Lisette, et al. Marlin-1 and Conventional Kinesin Link Gabab Receptors to the Cytoskeleton and Regulate Receptor Transport.. Mol. Cell. Neurosci. Jul 2007;35:501-12
Abstract
The cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABA(B) receptors (GABA(B)Rs) to the cytoskeleton have not been described. We recently identified Marlin-1, a protein that interacts with GABA(B)R1. Here, we explore the association of GABA(B)Rs and Marlin-1 to the cytoskeleton using a combination of biochemistry, microscopy and live cell imaging. Our results indicate that Marlin-1 is associated to microtubules and the molecular motor kinesin-I. We demonstrate that a fraction of Marlin-1 is mobile in dendrites of cultured hippocampal neurons and that mobility is microtubule-dependent. We also show that GABA(B)Rs interact robustly with kinesin-I and that intracellular membranes containing GABA(B)Rs are sensitive to treatments that disrupt a protein complex containing Marlin-1, kinesin-I and tubulin. Finally, we report that a kinesin-I mutant severely impairs receptor transport. We conclude that Marlin-1 and kinesin-1 link GABA(B)Rs to the tubulin cytoskeleton in neurons.
Mesh Headings (Keywords): Animals, Antineoplastic Agents, Cells, Cultured, Cytoskeleton, Embryo, Mammalian, Female, Green Fluorescent Proteins, Hippocampus, Kinesin, Mice, Mice, Inbred BALB C, Neurons, Nocodazole, Pregnancy, Protein Transport, RNA-Binding Proteins, Rats, Rats, Sprague-Dawley, Receptors, GABA, Time Factors, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 17532644
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