Development of a Simplified and Standardized Protocol with Potential for High-throughput for Sperm Cryopreservation in Zebrafish Danio Rerio.
From: Aquaculture Research Station, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Theriogenology
- Publish Date: Jul 2007
- ISSN: 0093-691X
- Volume: 68
- Issue: 2
- Pages: 128-36
- Medium: Print
- Language: English
- Citation (JAMA): Yang Huiping, Carmichael Carrie, Varga Zoltan M, et al. Development of a Simplified and Standardized Protocol with Potential for High-throughput for Sperm Cryopreservation in Zebrafish Danio Rerio.. Theriogenology Jul 2007;68:128-36
Abstract
Sperm cryopreservation offers potential for long-term storage of genetic resources. However, the current protocols for zebrafish Danio rerio are cumbersome and poorly reproducible. Our objective was to facilitate adoption of cryopreservation by streamlining methods from sperm collection through thawing and use. First, sperm activation was evaluated, and motility was completely inhibited when osmolality of the extender was >/=295-300mOsmol/kg. To evaluate cryoprotectant toxicity, sperm were incubated with dimethyl sulfoxide (DMSO), N,N-dimethyl acetamide (DMA), methanol, or glycerol at 5, 10, and 15% concentrations. Based on motility, DMSO, DMA, and methanol (
Mesh Headings (Keywords): Animals, Cryopreservation, Cryoprotective Agents, Male, Methanol, Osmolar Concentration, Sperm Motility, Spermatozoa, Temperature, Zebrafish
Check for Full Text / PubMed Unique Identifier (PMID): 17544099
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