Cannabidiolic-acid Synthase, the Chemotype-determining Enzyme in the Fiber-type Cannabis Sativa.
From: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan. taura@phar.kyushu-u.ac.jp
FEBS letters
- Publish Date: Jun 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 16
- Pages: 2929-34
- Medium: Print
- Language: English
- Citation (JAMA): Taura Futoshi, Sirikantaramas Supaart, Shoyama Yoshinari, et al. Cannabidiolic-acid Synthase, the Chemotype-determining Enzyme in the Fiber-type Cannabis Sativa.. FEBS Lett. Jun 2007;581:2929-34
Abstract
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Base Sequence, Cannabis, Cells, Cultured, Cloning, Molecular, Coenzymes, Intramolecular Oxidoreductases, Models, Biological, Molecular Sequence Data, Plant Components, Protein Binding, Spodoptera, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 17544411
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