An Antibody to Synaptotagmin I Facilitates Synaptic Transmission.
From: Department of Biological Sciences, Barnard College, Columbia University, 3009 Broadway, New York, NY 10027, USA. shua@barnard.edu
The European journal of neuroscience
- Publish Date: Jun 2007
- ISSN: 0953-816X
- Volume: 25
- Issue: 11
- Pages: 3217-25
- Medium: Print
- Language: English
- Citation (JAMA): Hua Shao-Ying, Teylan Merilee A, Cimenser Aylin, et al. An Antibody to Synaptotagmin I Facilitates Synaptic Transmission.. Eur. J. Neurosci. Jun 2007;25:3217-25
Abstract
Proper functioning of the nervous system requires precise control of neurotransmitter release. Synaptotagmin, a synaptic vesicle protein, is crucial for the temporal control of neurotransmitter release. The mechanism of synaptotagmin function is still under debate. To investigate the mechanism by which synaptotagmin controls neurotransmitter release, we injected an antibody of rat synaptotagmin I into a crayfish motor axon. We found that the antibody enhanced synaptic transmission at crayfish neuromuscular junctions by increasing the amplitude of the evoked synaptic response. This effect was antibody-dose dependent. The antibody also reduced the rise time of the synaptic potentials. These effects were accompanied by a reduction in the Hill coefficient for Ca(2+)-dependence of synaptic transmission. Our findings support the hypothesis that synaptotagmin inhibits neurotransmitter release in the absence of Ca(2+).
Mesh Headings (Keywords): Animals, Antibodies, Blotting, Western, Calcium, Catfishes, Dose-Response Relationship, Drug, Drug Interactions, Excitatory Postsynaptic Potentials, Microinjections, Neuromuscular Junction, Patch-Clamp Techniques, Rats, Synaptic Transmission, Synaptotagmin I
Check for Full Text / PubMed Unique Identifier (PMID): 17552990
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