Transcription Factor Nfy Globally Represses the Expression of the C. Elegans Hox Gene Abdominal-b Homolog Egl-5.
From: Graduate Program in Chinese Academy of Medical Sciences & Peking Union Medical College, PR China.
Developmental biology
- Publish Date: Aug 2007
- ISSN: 0012-1606
- Volume: 308
- Issue: 2
- Pages: 583-92
- Medium: Print
- Language: English
- Citation (JAMA): Deng Hansong, Sun Yinyan, Zhang Yuxia, et al. Transcription Factor Nfy Globally Represses the Expression of the C. Elegans Hox Gene Abdominal-b Homolog Egl-5.. Dev. Biol. Aug 2007;308:583-92
Abstract
The C. elegans Hox gene egl-5 (ortholog of Drosophila Abdominal-B) is expressed in multiple tissues in the tail region and is involved in tail patterning. In this study, we identify and clone the corresponding C. elegans orthologs of the components of the heterotrimeric transcription factor NFY, nfya-1, nfyb-1 and nfyc-1 and demonstrate that mutations in these components result in the ectopic expression of egl-5 outside of its normal expression domains. The NFYA-1 protein forms a complex with NFYB-1 and NFYC-1, specifically recognizing the CCAAT box. Mutating a CCAAT box in the proximal promoter of egl-5 also leads to the derepression of egl-5, suggesting a direct role for the NFY complex in the regulation of egl-5. In addition, we show that the NFY complex interacts with the MES-2/MES-6 PcG complex in Hox gene regulation. Thus, our studies unravel a physiological function of NFY in establishing the spatially restricted expression pattern of egl-5.
Mesh Headings (Keywords): Animals, Animals, Genetically Modified, Base Sequence, CCAAT-Binding Factor, Caenorhabditis elegans, Caenorhabditis elegans Proteins, DNA, Helminth, Gene Expression Regulation, Developmental, Genes, Helminth, Genes, Homeobox, Genome, Helminth, Homeodomain Proteins, Mutation, Promoter Regions (Genetics), Repressor Proteins, Transcription Factors
Check for Full Text / PubMed Unique Identifier (PMID): 17574230
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.