Effects of Caffeine on in Vivo and in Vitro Oocyte Maturation in Mice.
From: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang, Beijing, PR China.
Theriogenology
- Publish Date: Sep 2007
- ISSN: 0093-691X
- Volume: 68
- Issue: 4
- Pages: 640-5
- Medium: Print
- Language: English
- Citation (JAMA): Miao Yi-Liang, Shi Li-Hong, Lei Zi-Li, et al. Effects of Caffeine on in Vivo and in Vitro Oocyte Maturation in Mice.. Theriogenology Sep 2007;68:640-5
Abstract
The objective was to investigate, using a mouse model, the effects of caffeine on the number of ovulated oocytes, the rate of oocyte maturation, the susceptibility of oocytes to activating stimuli, spindle morphology, and distribution of cortical granules (CGs). Mice were given caffeine (150 mg/kg body weight ip) at various times relative to hCG (-2, 0, and +2h); in an in vitro study, 1, 5 or 10 mM caffeine was added to the maturation culture. Caffeine had no effect on the quality of oocytes in vivo maturation, but caffeine was detrimental to the quality of oocytes matured in vitro. Further studies are needed to determine caffeine concentration in follicles relative to that in culture medium.
Mesh Headings (Keywords): Animals, Caffeine, Central Nervous System Stimulants, Female, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Mice, Microscopy, Confocal, Mitotic Spindle Apparatus, Oocytes, Propidium, Superovulation
Check for Full Text / PubMed Unique Identifier (PMID): 17576000
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