Ginsenosides Promote Proliferation of Chicken Primordial Germ Cells Via Pkc-involved Activation of Nf-kappab.
From: College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
Cell biology international
- Publish Date: Oct 2007
- ISSN: 1065-6995
- Volume: 31
- Issue: 10
- Pages: 1251-6
- Medium: Print
- Language: English
- Citation (JAMA): Ge Chutian, Zhang Caiqiao, Ye Jian, et al. Ginsenosides Promote Proliferation of Chicken Primordial Germ Cells Via Pkc-involved Activation of Nf-kappab.. Cell Biol. Int. Oct 2007;31:1251-6
Abstract
The effect of ginsenosides on proliferation of chicken primordial germ cells (PGCs) was evaluated and involvement of nuclear factor (NF)-kappaB in the signaling pathway was investigated. PGCs were isolated from the genital ridge of 3.5-4 day embryos and cultured in Medium 199 supplemented with 5% FCS and 10 ng/ml LIF. PGCs subcultured on chicken embryonic fibroblast feeder were challenged with ginsenosides alone or in combination with PKC inhibitor H(7) or activator phorbol 12-myristate 13-acetate (PMA) for 24h. Moreover, the translocation of NF-kappaB and degradation level of IkappaBalpha were investigated by Western blot analysis. Results show that PGCs were identified by periodic acid-Schiff, alkaline phosphatase histochemistry as well as c-kit, SSEA-1 and Oct-4 immunocytochemistry. Treatment with ginsenosides at 1-100 microg/ml significantly increased the number and area of PGC colonies in a dose-dependent manner. However, this proliferating effect was obviously attenuated by combined treatment of H(7) (10(-7)-10(-5)M). Similarly, PKC staining of PGC colonies was more intensive after ginsenosides treatment compared with the control group. In addition, treatment with ginsenosides at 1-10 microg/ml stimulated the translocation of NF-kappaB (p65). However, the NF-kappaB translocation and the degradation of IkappaBalpha were significantly blocked by combined treatment with 10(-6)M H(7). These results indicated that ginsenosides promote proliferation of chicken PGCs through activation of PKC-involved NF-kappaB signaling pathway.
Mesh Headings (Keywords): Animals, Carcinogens, Cell Proliferation, Cells, Cultured, Chickens, Enzyme Activation, Germ Cells, Ginsenosides, I-kappa B Proteins, NF-kappa B, Protein Kinase C, Protein Transport, Signal Transduction, Tetradecanoylphorbol Acetate
Check for Full Text / PubMed Unique Identifier (PMID): 17582792
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