Enhancement of Ifn-gamma Production for Th1-cell Therapy Using Negatively Charged Liposomes Containing Phosphatidylserine.
From: Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Vaccine
- Publish Date: Jul 2007
- ISSN: 0264-410X
- Volume: 25
- Issue: 29
- Pages: 5256-62
- Medium: Print
- Language: English
- Citation (JAMA): Yotsumoto Satoshi, Kakiuchi Terutaka, Aramaki Yukihiko, et al. Enhancement of Ifn-gamma Production for Th1-cell Therapy Using Negatively Charged Liposomes Containing Phosphatidylserine.. Vaccine Jul 2007;25:5256-62
Abstract
We have developed an efficient method of interferon-gamma (IFN-gamma) induction for Th1-cell therapy. OVA (ovalbumin)-specific Th1 clone 42-6A cells cocultured with antigen presenting cells (APCs) from spleen resulted in high levels of OVA-specific IFN-gamma production by the treatment of phosphatidylserine (PS), but not phosphatidic acid (PA), liposomes-encapsulated OVA (OVA-liposomes). The IFN-gamma production was increased in a manner dependent on the PS content of the liposomes and inhibited by the addition of annexin V, a PS binding protein. Furthermore, coadministration of Th1 cells plus OVA-liposomes in mice strikingly enhanced IFN-gamma levels in serum and in spleen cells compared with that of Th1 cells plus OVA. In addition, serum levels of IL-12 p70 increased and ongoing OVA-specific IgE immune response was dramatically attenuated. These results first suggest that antigen delivery using negatively charged liposomes containing PS is very useful for the enhancement of IFN-gamma production in Th1-cell therapy.
Mesh Headings (Keywords): Animals, Antigen-Presenting Cells, Cells, Cultured, Coculture Techniques, Immunoglobulin E, Immunologic Factors, Interferon Type II, Interleukin-12, Liposomes, Mice, Mice, Inbred BALB C, Models, Animal, Ovalbumin, Phosphatidylserines, Th1 Cells
Check for Full Text / PubMed Unique Identifier (PMID): 17583394
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.