Three-dimensional Cell Culture and Tissue Engineering in a T-cup (Tissue Culture Under Perfusion).
From: Tissue Engineering, Laboratory 405, Departments of Surgery and of Research, Hebelstrasse 20, CH-4031 Basel, Switzerland.
Tissue engineering
- Publish Date: Aug 2007
- ISSN: 1076-3279
- Volume: 13
- Issue: 8
- Pages: 2021-8
- Medium: Print
- Language: English
- Citation (JAMA): Timmins Nicholas E, Scherberich Arnaud, Früh Jennifer-Annika, et al. Three-dimensional Cell Culture and Tissue Engineering in a T-cup (Tissue Culture Under Perfusion).. Tissue Eng. Aug 2007;13:2021-8
Abstract
The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds. The developed Tissue Culture Under Perfusion (T-CUP) bioreactor is based on the concept of controlled and confined alternating motion of scaffolds through a cell suspension or culture medium, as opposed to pumping of the fluid through the scaffolds. Via the T-CUP, articular chondrocytes and bone marrow stromal cells could be seeded into porous scaffolds of different compositions and architectures (chronOS, Hyaff-11, and Polyactive) at high efficiency (greater than 75%), uniformity (cells were well distributed throughout the scaffold pores), and viability (greater than 97%). Culture of articular chondrocytes seeded into 4-mm thick Polyactive scaffolds for 2 weeks in the T-CUP resulted in uniform deposition of cartilaginous matrix. Cultivation of freshly isolated human bone marrow nucleated cells seeded into ENGipore ceramic scaffolds for 19 days in the T-CUP resulted in stromal cell-populated constructs capable of inducing ectopic bone formation in nude mice. The T-CUP bioreactor represents an innovative approach to simple, efficient, and reliable 3D cell culture, and could be used either as a model to investigate mechanisms of tissue development or as a graft manufacturing system in the context of regenerative medicine.
Mesh Headings (Keywords): Animals, Bioreactors, Bone Marrow Cells, Bone Marrow Transplantation, Cartilage, Articular, Cattle, Cell Culture Techniques, Cells, Cultured, Chondrocytes, Humans, Mice, Mice, Nude, Perfusion, Tissue Culture Techniques, Tissue Engineering
Check for Full Text / PubMed Unique Identifier (PMID): 17590148
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.