Preliminary Studies on the Vitrification of Red Sea Bream (Pagrus Major) Embryos.
From: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
Theriogenology
- Publish Date: Sep 2007
- ISSN: 0093-691X
- Volume: 68
- Issue: 5
- Pages: 702-8
- Medium: Print
- Language: English
- Citation (JAMA): Ding F H, Xiao Z Z, Li J, et al. Preliminary Studies on the Vitrification of Red Sea Bream (Pagrus Major) Embryos.. Theriogenology Sep 2007;68:702-8
Abstract
The objective was to identify an appropriate cryoprotectant and protocol for vitrification of red sea bream (Pagrus major) embryos. The toxicity of five single-agent cryoprotectants, dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), glycerol (GLY), and methyl alcohol (MeOH), as well as nine cryoprotectant mixtures, were investigated by comparing post-thaw hatching rates. Two vitrifying protocols, a straw method and a solid surface vitrification method (copper floating over liquid nitrogen), were evaluated on the basis of post-thaw embryo morphology. Exposure to single-agent cryoprotectants (10% concentration for 15 min) was not toxic to embryos, whereas for higher concentrations (20 and 30%) and a longer duration of exposure (30 min), DMSO and PG were better tolerated than the other cryoprotectants. Among nine cryoprotectant mixtures, the combination of 20% DMSO+10% PG+10% MeOH had the lowest toxicity after exposure for 10 min or 15 min. High percentages of morphologically intact embryos, 50.6+/-16.7% (mean+/-S.D.) and 77.8+/-15.5%, were achieved by the straw vitrifying method (20.5% DMSO+15.5% acetamide+10% PG, thawing at 43 degrees C and washing in 0.5M sucrose solution for 5 min) and by the solid surface vitrification method (40% GLY, thawing at 22 degrees C and washing in 0.5M sucrose solution for 5 min). After thawing, morphological changes in the degenerated embryos included shrunken yolks and ruptured chorions. Furthermore, thawed embryos that were morphologically intact did not consistently survive incubation.
Mesh Headings (Keywords): Animals, Cryopreservation, Cryoprotective Agents, Drug Combinations, Embryo, Nonmammalian, Models, Biological, Pilot Projects, Sea Bream
Check for Full Text / PubMed Unique Identifier (PMID): 17606292
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.