Identification of Candidate Transcriptional Modulators Involved in Successful Regeneration After Nerve Injury.
From: Department of Molecular and Cellular Neurobiology, Center for Neurogenomics & Cognitive Research, Faculty of Earth and Life Sciences, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands. stam@salk.edu
The European journal of neuroscience
- Publish Date: Jun 2007
- ISSN: 0953-816X
- Volume: 25
- Issue: 12
- Pages: 3629-37
- Medium: Print
- Language: English
- Citation (JAMA): Stam Floor J, MacGillavry Harold D, Armstrong Nicola J, et al. Identification of Candidate Transcriptional Modulators Involved in Successful Regeneration After Nerve Injury.. Eur. J. Neurosci. Jun 2007;25:3629-37
Abstract
Successful regeneration of injured neurons requires a complex molecular response that involves the expression, modification and transport of a large number of proteins. The identity of neuronal proteins responsible for the initiation of regenerative neurite outgrowth is largely unknown. Dorsal root ganglion (DRG) neurons display robust and successful regeneration following lesion of their peripheral neurite, whereas outgrowth of central neurites is weak and does not lead to functional recovery. We have utilized this differential response to gain insight in the early transcriptional events associated with successful regeneration. Surprisingly, our study shows that peripheral and central nerve crushes elicit very distinct transcriptional activation, revealing a large set of novel genes that are differentially regulated within the first 24 h after the lesion. Here we show that Ankrd1, a gene known to act as a transcriptional modulator, is involved in neurite outgrowth of a DRG neuron-derived cell line as well as in cultured adult DRG neurons. This gene, and others identified in this study, may be part of the transcriptional regulatory module that orchestrates the onset of successful regeneration.
Mesh Headings (Keywords): Animals, Cells, Cultured, Female, Ganglia, Spinal, Gene Expression Profiling, Gene Expression Regulation, In Situ Hybridization, Male, Nerve Regeneration, Neurons, Nuclear Proteins, Rats, Rats, Sprague-Dawley, Rats, Wistar, Repressor Proteins, Sciatic Neuropathy, Spinal Cord Injuries, Transcription Factors, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 17610582
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.