Monitoring of Microbiological Water Quality by Real-time Pcr.
From: River Basin Research Centre, Gifu University, 1-1, Yanagido, Gifu 501-1193, Japan.
Environmental technology
- Publish Date: May 2007
- ISSN: 0959-3330
- Volume: 28
- Issue: 5
- Pages: 545-53
- Medium: Print
- Language: English
- Citation (JAMA): Zhou W, Kageyama K, Li F, et al. Monitoring of Microbiological Water Quality by Real-time Pcr.. Environ Technol May 2007;28:545-53
Abstract
A new method was developed to evaluate the microbiological water quality. Deoxyribonucleic acid of water-borne bacteria was extracted and quantified using real-time polymer chain reaction detection system with a selected universal primer set. Quantification of the deoxyribonucleic acid in environmental water samples is independent of the culture condition, nutrient condition, or the bacterial metabolic states and can reflect the relatively small environmental changes which can not be detected through the other parameters. Therefore, this method can well represent the microbiological water quality. Compared with the conventional plate count method, deoxyribonucleic acid quantification has higher sensitivity, precision and reliability. The relationship between concentration of bacterial deoxyribonucleic acid and other water quality parameters was examined. Concentration of deoxyribonucleic acid was not well correlated with the plate count number, turbidity or other physical and chemical parameters.
Mesh Headings (Keywords): Bacteria, Bacterial Physiology, DNA, Bacterial, Polymerase Chain Reaction, Sensitivity and Specificity, Water Microbiology, Water Supply
Check for Full Text / PubMed Unique Identifier (PMID): 17615963
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