Cell-surface Interactions of Rat Tooth Germ Cells on Various Biomaterials.
From: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan.
Journal of biomedical materials research. Part A
- Publish Date: Oct 2007
- ISSN: 1549-3296
- Volume: 83
- Issue: 1
- Pages: 241-8
- Medium: Print
- Language: English
- Citation (JAMA): Chen Rung-Shu, Chen Yi-Jane, Chen Min-Huey, et al. Cell-surface Interactions of Rat Tooth Germ Cells on Various Biomaterials.. Oct 2007;83:241-8
Abstract
This is the first study to explore the effect of biomaterial on tooth germ cell adhesion and proliferation in vitro. The purpose of this study is to evaluate the effects of cell-surface interactions of tooth germ cells on biomaterials with various surface hydrophilicities. The biomaterials used in this study included polyvinyl alcohol (PVA), poly(lactic-co-glycolic acid) (PLGA), poly(ethylene-co-vinyl alcohol; EVAL), and polyvinylidene fluoride (PVDF). Cell morphology was observed by photomicroscopy. Cell growth was assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction activity and the characteristic expression of amelogenin and collagen type I in tooth germ cells was investigated using immunocytochemistry. The results indicated that adhesion and proliferation of tooth germ cells to biomaterials with moderate hydrophilicity/hydrophobicity was superior compared to most hydrophobic material PVDF or mosthydrophilic material PVA in this study. Cellular adhesion and proliferation was evident on all tested biomaterials except PVA. The cell spheroids on PVA appeared not to be proliferated and remained as well as reattachable to tissue culture plates. In conclusion, biomaterials with moderate hydrophilicity are suitable for adhesion and proliferation of tooth germ cells. The material PVA may be a good biomaterial for maintaining tooth germ cells in three-dimensional biological restoration.
Mesh Headings (Keywords): Animals, Biocompatible Materials, Cell Adhesion, Cell Membrane, Cell Separation, Cells, Cultured, Formazans, Hydrophobicity, Immunohistochemistry, Oxidation-Reduction, Rats, Rats, Wistar, Surface Properties, Tetrazolium Salts, Tooth Germ
Check for Full Text / PubMed Unique Identifier (PMID): 17618501
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