Ifn-gamma-induced Protein 10 is a Novel Biomarker of Rhinovirus-induced Asthma Exacerbations.
From: Brooke Laboratories, University of Southampton, Southampton, United Kingdom. peter.wark@hnehealth.nsw.gov.au
The Journal of allergy and clinical immunology
- Publish Date: Sep 2007
- ISSN: 0091-6749
- Volume: 120
- Issue: 3
- Pages: 586-93
- Medium: Print
- Language: English
- Citation (JAMA): Wark Peter A B, Bucchieri Fabio, Johnston Sebastian L, et al. Ifn-gamma-induced Protein 10 is a Novel Biomarker of Rhinovirus-induced Asthma Exacerbations.. J. Allergy Clin. Immunol. Sep 2007;120:586-93
Abstract
BACKGROUND: Rhinovirus-induced acute asthma is the most frequent trigger for asthma exacerbations. OBJECTIVE: We assessed which inflammatory mediators were released from bronchial epithelial cells (BECs) after infection with rhinovirus and then determined whether they were also present in subjects with acute virus-induced asthma, with the aim to identify a biomarker or biomarkers for acute virus-induced asthma. METHODS: BECs were obtained from bronchial brushings of steroid-naive asthmatic subjects and healthy nonatopic control subjects. Cells were infected with rhinovirus 16. Inflammatory mediators were measured by means of flow cytometry with a cytometric bead array. Subjects with acute asthma and virus infection were recruited; they were characterized clinically by using lung function tests and had blood taken to measure the inflammatory mediators identified as important by the BEC experiments. RESULTS: IFN-gamma-induced protein 10 (IP-10) and RANTES were released in the greatest quantities, followed by IL-6, IL-8, and TNF-alpha. Dexamethasone treatment of BECs only partially suppressed IP-10 and TNF-alpha but was more effective at suppressing RANTES, IL-6, and IL-8. In acute clinical asthma serum IP-10 levels were increased to a greater extent in those with acute virus-induced asthma (median of 604 pg/mL compared with 167 pg/mL in those with non-virus-induced acute asthma, P < .01). Increased serum IP-10 levels were predictive of virus-induced asthma (odds ratio, 44.3 [95% CI, 3.9-100.3]). Increased serum IP-10 levels were strongly associated with more severe airflow obstruction (r = -0.8; P < .01). CONCLUSIONS: IP-10 release is specific to acute virus-induced asthma. CLINICAL IMPLICATIONS: Measurement of serum IP-10 could be used to predict a viral trigger to acute asthma.
Mesh Headings (Keywords): Adolescent, Adult, Aged, Anti-Inflammatory Agents, Asthma, Biological Markers, Cells, Cultured, Chemokine CCL5, Chemokine CXCL10, Chemokines, CXC, Dexamethasone, Epithelial Cells, Flow Cytometry, Humans, Interleukin-6, Interleukin-8, Lung, Middle Aged, Picornaviridae Infections, Rhinovirus, Tumor Necrosis Factor-alpha
Check for Full Text / PubMed Unique Identifier (PMID): 17628646
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