Induction of T-cell Response by a Dna Vaccine Encoding a Novel Hla-a*0201 Severe Acute Respiratory Syndrome Coronavirus Epitope.
From: Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
Vaccine
- Publish Date: Aug 2007
- ISSN: 0264-410X
- Volume: 25
- Issue: 32
- Pages: 6070-7
- Medium: Print
- Language: English
- Citation (JAMA): Cheung Ying-Kit, Cheng Samuel Chak-Sum, Sin Fion Wan-Yee, et al. Induction of T-cell Response by a Dna Vaccine Encoding a Novel Hla-a*0201 Severe Acute Respiratory Syndrome Coronavirus Epitope.. Vaccine Aug 2007;25:6070-7
Abstract
The severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV N) is one of the major targets for SARS vaccine due to its high potency in triggering immune responses. In this study, we have identified a novel HLA-A*0201 restricted epitope, N220 (LALLLLDRL), of the SARS-CoV N-protein through bioinformatics analysis. The N-protein peptide N220 shows a high binding affinity towards human MHC class I in T2-cells, and is capable of activating cytotoxic T-cells in human peripheral blood mononuclear cells (PBMCs). The application of using the N220 peptide sequence with a single-chain-trimer (SCT) approach to produce a potential DNA vaccine candidate was investigated in HLA-A2.1K(b) transgenic mice. Cytotoxicity assay clearly showed that the T-cells obtained from the vaccinated animals were able to kill the N-protein expressing cells with a cytotoxicity level of 86% in an effector cells/target cells ratio of 81:1 one week after the last vaccination, which is significantly higher than other N-protein peptides previously described. The novel immunogenic N-protein peptide revealed in the present study provides valuable information for therapeutic SARS vaccine design.
Mesh Headings (Keywords): Animals, CD8-Positive T-Lymphocytes, Cell Line, Epitopes, T-Lymphocyte, HLA-A Antigens, Humans, Mice, Mice, Transgenic, Nucleocapsid Proteins, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, T-Lymphocytes, Cytotoxic, Vaccines, DNA, Viral Vaccines
Check for Full Text / PubMed Unique Identifier (PMID): 17629360
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