A Functional Ubiquitin-specific Protease Embedded in the Large Tegument Protein (Orf64) of Murine Gammaherpesvirus 68 is Active During the Course of Infection.
From: Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA.
Journal of virology
- Publish Date: Oct 2007
- ISSN: 0022-538X
- Volume: 81
- Issue: 19
- Pages: 10300-9
- Medium: Print
- Language: English
- Citation (JAMA): Gredmark Sara, Schlieker Christian, Quesada Victor, et al. A Functional Ubiquitin-specific Protease Embedded in the Large Tegument Protein (Orf64) of Murine Gammaherpesvirus 68 is Active During the Course of Infection.. J. Virol. Oct 2007;81:10300-9
Abstract
All herpesviruses contain a ubiquitin (Ub)-specific cysteine protease domain embedded within their large tegument protein, based on homology with the corresponding sequences of UL36 from herpes simplex virus type 1 and M48 from murine cytomegalovirus. This type of activity has yet to be demonstrated for cells infected with a gammaherpesvirus. By activity-based profiling, we show that the large tegument protein of murine gammaherpesvirus (MHV-68) ORF64 (273 kDa) is a functional deubiquitinating protease, as assessed by tandem mass spectrometry of adducts in extracts from MHV-68-infected cells that had been labeled with ubiquitin vinylmethylester, a ubiquitin-based active site-directed probe. The recombinantly expressed amino-terminal segment of ORF64 displays deubiquitinating activity toward Ub C-terminal 7-amido-4-methylcoumarin in vitro. The findings reported here for MHV-68 ORF64 extend those made for the alpha- and betaherpesvirus families and are consistent with an important, conserved enzymatic function of the tegument protein.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Cell Line, Endopeptidases, Fibroblasts, Gammaherpesvirinae, Herpesviridae Infections, Mice, Molecular Sequence Data, Open Reading Frames, Phylogeny, Protein Conformation, Substrate Specificity, Viral Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 17634221
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