Caloric Restriction and Life Span Determination of Yeast Cells.
From: Pharmacology Department, Harvard Medical School, Boston, MA, USA.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2007
- ISSN: 1064-3745
- Volume: 371
- Issue:
- Pages: 97-109
- Medium: Print
- Language: English
- Citation (JAMA): Medvedik Oliver, Sinclair David A, et al. Caloric Restriction and Life Span Determination of Yeast Cells.. Methods Mol. Biol. 2007;371:97-109
Abstract
The diet known as caloric restriction (CR) has been known for 70 yr to extend the life span of rodents (1). CR can also extend life span in a broad range of other species as well, from unicellular organisms (2,3), to invertebrates (4) and most likely primates, as well (5). The budding yeast Saccharomyces cerevisiae is a useful model for the study of pathways that determine life span in response to dietary intake. Here, we describe how to calorically restrict yeast, the methods used to determine the replicative life span of single yeast “mother” cells and measure recombination frequency at the rDNA locus, and how to isolate and analyze the circular forms of DNA known as extrachromosomal rDNA circles (ERCs), which are a major cause of aging in S. cerevisiae (6-8).
Mesh Headings (Keywords): Animals, Caloric Restriction, DNA, Fungal, DNA, Ribosomal, Humans, Longevity, Models, Biological, Quantitative Trait Loci, Recombination, Genetic, Saccharomyces cerevisiae
Check for Full Text / PubMed Unique Identifier (PMID): 17634577
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