Expression and Function of Vascular Endothelial Growth Factor Receptors (Flt-1 and Flk-1) in Vascular Adventitial Fibroblasts.
From: Laboratory of Vascular Biology, Institute of Health Science Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Journal of molecular and cellular cardiology
- Publish Date: Sep 2007
- ISSN: 0022-2828
- Volume: 43
- Issue: 3
- Pages: 292-300
- Medium: Print
- Language: English
- Citation (JAMA): Jin Xin, Ge Xiaoli, Zhu Ding-liang, et al. Expression and Function of Vascular Endothelial Growth Factor Receptors (Flt-1 and Flk-1) in Vascular Adventitial Fibroblasts.. J. Mol. Cell. Cardiol. Sep 2007;43:292-300
Abstract
Vascular endothelial growth factor receptors (VEGFRs) are previously considered to exist exclusively in endothelial cells. However, little is known if the receptors are expressed in other non-endothelial cells. In this study, we measured activation of two VEGFRs, Flk-1 and Flt-1, and their biological functions in cultured adventitial fibroblasts and injured rat carotid injury arteries induced by balloon angioplasty. Our results indicated that Flt-1, but not Flk-1, existed in adventitial fibroblasts. Angiotensin II increased Flt-1 protein expression in a time- and concentration-dependent manner. Adventitial fibroblast migration stimulated by vascular endothelial growth factor (VEGF) and placental growth factor (PIGF) required Flt-1 expression. The Flt-1-induced adventitial fibroblast migration was blocked by anti-Flt-1 neutralizing antibody and soluble VEGFR1 protein (sFlt-1). However, Flt-1 activation did not enhance cell proliferation. In addition, Flt-1 expression was significantly increased in the neointima and adventitia in injured rat carotid arteries. We concluded that functional expression of Flt-1 in adventitial fibroblasts might be an important mediator in the pathogenesis of vascular remodeling after arterial injury.
Mesh Headings (Keywords): Angiotensin II, Animals, Aorta, Thoracic, Arteries, Carotid Arteries, Cell Movement, Cells, Cultured, Connective Tissue, Dose-Response Relationship, Drug, Fibroblasts, Immunohistochemistry, Male, Rats, Rats, Sprague-Dawley, Time Factors, Tunica Intima, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-1, Vascular Endothelial Growth Factor Receptor-2
Check for Full Text / PubMed Unique Identifier (PMID): 17651752
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