The Golgi-associated Protein Grasp is Required for Unconventional Protein Secretion During Development.
From: Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Cell
- Publish Date: Aug 2007
- ISSN: 0092-8674
- Volume: 130
- Issue: 3
- Pages: 524-34
- Medium: Print
- Language: English
- Citation (JAMA): Kinseth Matthew A, Anjard Christophe, Fuller Danny, et al. The Golgi-associated Protein Grasp is Required for Unconventional Protein Secretion During Development.. Cell Aug 2007;130:524-34
Abstract
During Dictyostelium development, prespore cells secrete acyl-CoA binding protein (AcbA). Upon release, AcbA is processed to generate a peptide called spore differentiation factor-2 (SDF-2), which triggers terminal differentiation of spore cells. We have found that cells lacking Golgi reassembly stacking protein (GRASP), a protein attached peripherally to the cytoplasmic surface of Golgi membranes, fail to secrete AcbA and, thus, produce inviable spores. Surprisingly, AcbA lacks a signal sequence and is not secreted via the conventional secretory pathway (endoplasmic reticulum-Golgi-cell surface). GRASP is not required for conventional protein secretion, growth, and the viability of vegetative cells. Our findings reveal a physiological role of GRASP and provide a means to understand unconventional secretion and its role in development.
Mesh Headings (Keywords): Adenylate Cyclase, Amino Acid Sequence, Animals, Dictyostelium, Golgi Apparatus, Membrane Proteins, Molecular Sequence Data, Protozoan Proteins, Spores, Protozoan
Check for Full Text / PubMed Unique Identifier (PMID): 17655921
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