Presence of Robust Circadian Clock Oscillation Under Constitutive Over-expression of Mcry1 in Rat-1 Fibroblasts.
From: COE Unit of Circadian Systems, Department of Biological Sciences, Nagoya University Graduate School of Science, Nagoya 464-8602, Japan.
FEBS letters
- Publish Date: Aug 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 21
- Pages: 4098-102
- Medium: Print
- Language: English
- Citation (JAMA): Yamanaka Iori, Koinuma Satoshi, Shigeyoshi Yasufumi, et al. Presence of Robust Circadian Clock Oscillation Under Constitutive Over-expression of Mcry1 in Rat-1 Fibroblasts.. FEBS Lett. Aug 2007;581:4098-102
Abstract
In mammals, mCRY proteins are essential and are major negative elements in circadian feedback loops. In this study, robust circadian clock oscillation was present even under conditions with constitutive over-expression of mCry1 in rat-1 cells. Rat-1 cells were produced to stably express mPer2 promoter-driven luciferase reporter, in which mCry1 was overexpressed under a tetracycline-dependent gene expression (Tet-On) system. Using these cells, we show that circadian clock oscillations in rat-1 fibroblasts persist when the mCRY1 protein constitutively accumulates in the nuclei.
Mesh Headings (Keywords): Active Transport, Cell Nucleus, Animals, Biological Clocks, Cell Cycle Proteins, Cell Line, Cell Nucleus, Circadian Rhythm, Fibroblasts, Flavoproteins, Gene Expression, Genes, Reporter, Luciferases, Nuclear Proteins, Rats
Check for Full Text / PubMed Unique Identifier (PMID): 17686474
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