Hiv-1(89.6) Gag Expressed from a Replication Competent Hsv-1 Vector Elicits Persistent Cellular Immune Responses in Mice.
From: Department of Medicine, The University of Alabama at Birmingham, Birmingham, AL 35294, United States.
Vaccine
- Publish Date: Sep 2007
- ISSN: 0264-410X
- Volume: 25
- Issue: 37-38
- Pages: 6764-73
- Medium: Print
- Language: English
- Citation (JAMA): Parker Scott D, Rottinghaus Scott T, Zajac Allan J, et al. Hiv-1(89.6) Gag Expressed from a Replication Competent Hsv-1 Vector Elicits Persistent Cellular Immune Responses in Mice.. Vaccine Sep 2007;25:6764-73
Abstract
We have constructed a replication competent, gamma(1)34.5-deleted herpes simplex virus type-1 (HSV-1) vector (J200) that expresses the gag gene from human immunodeficiency virus type-1, primary isolate 89.6 (HIV-1(89.6)), as a candidate vaccine for HIV-1. J200 replicates in vitro, resulting in abundant Gag protein production and accumulation in the extracellular media. Immunization of Balb/c mice with a single intraperitoneal injection of J200 elicited strong Gag-specific CD8 responses, as measured by intracellular IFN-gamma staining and flow cytometry analysis. Responses were highest between 6 weeks and 4 months, but persisted at 9 months post-immunization, the last time-point evaluated. These data highlight the potential utility of neuroattenuated, replication competent HSV-1 vectors for delivery of HIV-1 immunogens.
Mesh Headings (Keywords): Animals, CD8-Positive T-Lymphocytes, Cell Line, Cercopithecus aethiops, Female, Gene Expression Regulation, Viral, Gene Products, gag, Genetic Vectors, HIV-1, Immunization, Mice, Mice, Inbred BALB C, Virus Replication
Check for Full Text / PubMed Unique Identifier (PMID): 17706843
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