Scrapper-dependent Ubiquitination of Active Zone Protein Rim1 Regulates Synaptic Vesicle Release.
From: Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
Cell
- Publish Date: Sep 2007
- ISSN: 0092-8674
- Volume: 130
- Issue: 5
- Pages: 943-57
- Medium: Print
- Language: English
- Citation (JAMA): Yao Ikuko, Takagi Hiroshi, Ageta Hiroshi, et al. Scrapper-dependent Ubiquitination of Active Zone Protein Rim1 Regulates Synaptic Vesicle Release.. Cell Sep 2007;130:943-57
Abstract
Little is known about how synaptic activity is modulated in the central nervous system. We have identified SCRAPPER, a synapse-localized E3 ubiquitin ligase, which regulates neural transmission. SCRAPPER directly binds and ubiquitinates RIM1, a modulator of presynaptic plasticity. In neurons from Scrapper-knockout (SCR-KO) mice, RIM1 had a longer half-life with significant reduction in ubiquitination, indicating that SCRAPPER is the predominant ubiquitin ligase that mediates RIM1 degradation. As anticipated in a RIM1 degradation defect mutant, SCR-KO mice displayed altered electrophysiological synaptic activity, i.e., increased frequency of miniature excitatory postsynaptic currents. This phenotype of SCR-KO mice was phenocopied by RIM1 overexpression and could be rescued by re-expression of SCRAPPER or knockdown of RIM1. The acute effects of proteasome inhibitors, such as upregulation of RIM1 and the release probability, were blocked by the impairment of SCRAPPER. Thus, SCRAPPER has an essential function in regulating proteasome-mediated degradation of RIM1 required for synaptic tuning.
Mesh Headings (Keywords): Animals, Brain, Cells, Cultured, Enzyme Inhibitors, Excitatory Postsynaptic Potentials, GTP-Binding Proteins, Genotype, Half-Life, Kinetics, Leupeptins, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Neuronal Plasticity, Neurons, Phenotype, Presynaptic Terminals, Proteasome Endopeptidase Complex, Protein Processing, Post-Translational, RNA, Messenger, Rats, Rats, Wistar, Synapses, Synaptic Transmission, Synaptic Vesicles, Transfection, Ubiquitin, Ubiquitin-Protein Ligases
Check for Full Text / PubMed Unique Identifier (PMID): 17803915
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