Time-dependent Alteration in Cromakalim-induced Relaxation of Corpus Cavernosum from Streptozocin-induced Diabetic Rats.
From: Department of Pharmacology, School of Medicine, Medical Sciences / University of Tehran, PO Box: 13145-784, Tehran, Iran.
Life sciences
- Publish Date: Sep 2007
- ISSN: 0024-3205
- Volume: 81
- Issue: 12
- Pages: 960-9
- Medium: Print
- Language: English
- Citation (JAMA): Ghasemi Mehdi, Sadeghipour Hamed, Asadi Shahrzad, et al. Time-dependent Alteration in Cromakalim-induced Relaxation of Corpus Cavernosum from Streptozocin-induced Diabetic Rats.. Life Sci. Sep 2007;81:960-9
Abstract
The purpose of the present study was to investigate the relaxant responses to the ATP-sensitive potassium (K(ATP)) channel opener cromakalim in corpus cavernosum strips from 1-, 2-, 4-, 6-, and 8-week streptozocin-induced diabetic rats. Cromakalim (1 nM-0.1 mM) produced concentration-dependent relaxation in phenylephrine (7.5 microM)-precontracted isolated rat corporal strips. Compared with age-matched control animals, a significant enhancement in cromakalim-induced relaxation of corpus cavernosum was observed in 2-week diabetic animals, whereas the relaxant responses to cromakalim were decreased in 6-and 8-week diabetic animals. However, the cromakalim-induced relaxation was not altered in either 1-week or 4-week rat corporal strips in comparison with corresponding age-matched non-diabetic groups. Preincubation with the K(ATP) channel blocker glibenclamide (10 microM) significantly inhibited the cromakalim-induced relaxation in both non-diabetic and diabetic rat corpus cavernosum, but neither the voltage-dependent K(+) channel (K(V)) antagonist 4-aminopyridine (1 mM) nor the calcium-activated K(+) channel (K(Ca)) antagonist charybdotoxin (0.1 microM) had significant effect on cromakalim-induced relaxation in both control and diabetic rat corporal strips. Relaxation responses to the nitric oxide donor sodium nitroprusside (1 nM-0.1 mM) in diabetic rat corpus cavernosum were similar to that of age-matched controls. These data demonstrated that the relaxant responses to cromakalim were altered in diabetic cavernosal strips in a time dependent manner, suggesting that the period of diabetes mellitus may play a key role in the K(ATP) channels function in rat corpus cavernosum.
Mesh Headings (Keywords): Animals, Cromakalim, Diabetes Mellitus, Experimental, Dose-Response Relationship, Drug, Glyburide, Male, Muscle Relaxation, Penis, Potassium Channels, Rats, Rats, Sprague-Dawley, Sodium-Potassium-Exchanging ATPase, Streptozocin, Time Factors
Check for Full Text / PubMed Unique Identifier (PMID): 17825847
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