Streptozotocin-induced Diabetes Increases the Interaction of Bad/Bcl-xl and Decreases the Binding of Pbad/14-3-3 in Rat Testis.
From: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 900 Gajwa-dong, Jinju 660-701, South Korea. pokoh@gsnu.ac.kr
Life sciences
- Publish Date: Sep 2007
- ISSN: 0024-3205
- Volume: 81
- Issue: 13
- Pages: 1079-84
- Medium: Print
- Language: English
- Citation (JAMA): Koh Phil-Ok, et al. Streptozotocin-induced Diabetes Increases the Interaction of Bad/Bcl-xl and Decreases the Binding of Pbad/14-3-3 in Rat Testis.. Life Sci. Sep 2007;81:1079-84
Abstract
Sexual dysfunction is frequently associated with diabetes in males. The present study was designed to evaluate whether streptozotocin-induced diabetes increases apoptotic cell death in rat testis through the regulation of Bcl-2 family proteins. Diabetes was induced by a single intravenous injection of streptozotocin (40 mg/kg body weight) and testis samples were collected after 3 months. The number of positive cells for TUNEL histochemistry was significantly increased in the testicular germ cells of the diabetic group, compared to those of control. The levels of Bcl-2 and Bcl-X(L), anti-apoptotic proteins, were decreased in the diabetic group. In contrast, the levels of Bax and Bad, pro-apoptotic factors, were increased in the diabetic group, compared with the control group. Moreover, the diabetic condition increased the interaction of Bad and Bcl-X(L), and decreased the binding of pBad and 14-3-3. 14-3-3 acts as an anti-apoptotic factor through interaction with Bad. Our findings suggest that streptozotocin-induced diabetes increases apoptotic cell death in testis tissue through the up-and down-regulation of Bcl-2 family proteins and the interaction of Bad and Bcl-X(L).
Mesh Headings (Keywords): 14-3-3 Proteins, Animals, Apoptosis, Cell Death, Diabetes Mellitus, Experimental, Gene Expression Regulation, Germ Cells, Inhibitor of Apoptosis Proteins, Male, Protein Binding, Rats, Rats, Sprague-Dawley, Streptozocin, Testis, bcl-Associated Death Protein, bcl-X Protein
Check for Full Text / PubMed Unique Identifier (PMID): 17870134
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